Eye Movement Image Analysis For Guinea Pig

Takuo Ikeda

Department of Otolaryngology, Yamaguchi University School of Medicine

E-mail: ikeda-t@yamaguchi-u.ac.jp

We devised a new eye movement image analysis technique for recording the horizontal vestibulo-ocular reflex in the guinea pig. For automatic analysis of guinea pig eye movement, we created a macro for use with the public domain software NIH Image (http://rsb.info.nih.gov/nih-image/). This macro was written in the NIH Image Pascal-like programming language.

　

　　　　 Eye movements of the guinea pig were recorded on videotape in the dark with the infrared CCD camera. Video images (320 x 240 pixels) were captured on a PC (Power Mac G4, Apple Computer) at 30 frames/sec.

　　　　 After capturing eye movement, unnecessary areas were removed from the images, and the threshold value for the pupils was set.

　　　　 After the threshold was set to provide for clear outlines of the pupil, the X-Y center of the pupil was analyzed, and the horizontal components and velocity of eye movements were calculated. During analysis, the X-Y center of the pupil was analyzed as the X-Y coordinates of the center of the best fitting ellipse. The data was calibrated by measuring the eyeball and pupil diameters of 10 extracted guinea pig bulbus oculi and taking the mean values.

　

Download

Macro-GineaPig(This file is compressed as a zip file.)

TestImage(This file is compressed as a zip file.)

NIH Image v1.62 Image

http://rsb.info.nih.gov/nih-image/

Preferences