1. PERSONAL DETAILS

Name:   Dai Mitsushima
Date of birth:  14th March 1965 (Osaka, JAPAN)
Nationality:  JAPAN
Work Address: Department of Physiology, Yamaguchi University Graduate School of Medicine
Web page: http://ds.cc.yamaguchi-u.ac.jp/~seiri2/e-index.html

1990    Bachelor of Veterinary Medicine (The University of Tokyo, Japan)
1996    Doctor of Veterinary Medicine (The University of Tokyo, Japan)

Japan Neuroendocrine Society (2001 - present)
The Japan Neuroscience Society (1994 - present)
Physiological Society of Japan (1994 - present)
The Japanese Society of Veterinary Science (1988 - present)
The Japan Society of Reproductive Endocrinology (2001 - present)
Society for Neuroscience (1997 - present)
American Association for the Advancement of Science (2004 - present)
Editorial board in Endocrinology Studies: Pavia, Italy. (2011 - present)
Editorial board in Hormonal Studies: Bedfordshire, UK. (2012 - present)
Editorial board in Neurotransmitter: North Post Oak, Texas, USA. (2014 - present)
Editorial board in Chronicles of Science: Johns Creek, Georgia, USA. (2014 - present)

Promotion Award of the Physiological Society of Japan (2000)
Promotion Award of the Japan Neuroscience Society (2002)
Medical Award of Yokohama City University (2003)
Listed in "Marquis Who's Who in the World." (2004)
Listed in "Marquis Who's Who in Science and Engineering." (2005)
Kawakami Award of the Japan Neuroendocrine Society (2005)
Sojinkai Prize of Yamaguchi University (2014)
Janson Award of the University of Tokyo (2016)

1990-1993     Research Associate, University of Wisconsin, National Primate Research Center, Madison, WI, USA.
1993              Honorary Fellow, University of Wisconsin, National Primate Research Center, Madison, WI, USA
1994-2002     Lecturer, Yokohama City University Graduate School of Medicine.
2002-2005     Assistant Professor, Yokohama City University Graduate School of Medicine.
2005-2012     Associate Professor, Yokohama City University Graduate School of Medicine.
2011-2012     Research Professor, Kanagawa Dental University.

Professor, Department of Physiology,
Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.

Sakimoto Y, Kida H, Mitsushima D.
Temporal dynamics of learning-promoted synaptic diversity in CA1 pyramidal neurons.
FASEB J, in press, 2019.

Sakimoto Y, Mizuno J, Kida H, Kamiya Y, Ono Y, Mitsushima D.
Learning promotes subfield-specific synaptic diversity in hippocampal CA1 neurons.
Cerebral Cortex, 29: 2183-2195, doi:10.1093/cercor/bhz022, 2019.

Yamamoto Y, Kida H, Kagawa Y, Yasumoto Y, Miyazaki H, Islam A, Ogata M, Yanagawa Y, Mitsushima D,
Fukunaga K, Owada Y. FABP3 in the anterior cingulate cortex modulates the methylation status of the glutamic
acid decarboxylase67 promoter region.
J Neurosci, 38: 10411-10423; doi: https://doi.org/10.1523/JNEUROSCI.1285-18.2018.

Nomura S, Kida H, Hirayama Y, Imoto H, Inoue T, Moriyama H, Mitsushima D, Suzuki M.
Reduction of spike generation frequency by cooling in brain slices from rats and from patients with epilepsy.
J Cereb Blood Flow Metab, doi:10.1177/0271678X18795365. 2018.

Kida H, Mitsushima D.
Mechanisms of motor learning mediated by synaptic plasticity in rat primary motor cortex.
Neurosci Res, doi.org/10.1016/j.neures.2017.09.008. invited review article, 2017.

Kida H, Sakimoto Y, Mitsushima D. Slice patch-clamp technique for analyzing learning-induced plasticity.
J Vis Exp, e55876, doi:10.3791/55876, invited from editor, 2017.

Mitsushima D. Synaptic diversity and quantification of intra-hippocampal contextual memory.
Neurol Neurother 2(2): 000113, invited review, 2017.

Mitsushima D. Learning-induced synaptic diversity and quantification of intra-hippocampal entropy.
J Psychol Clin Psychiatry, 8(1): 00471, doi: 10.15406/jpcpy.2017.08.00471, invited review, 2017.

Sakimoto Y, Mitsushima D. Examination of configural association theory and conflict resolution model through
hippocampal theta activity.
Neurotransmitter, 3: e1478, doi:10.14800/nt.1478, invited review, 2016.

Kida H, Tsuda Y, Ito N, Yamamoto Y, Owada Y, Kamiya Y, Mitsushima D.
Motor training promotes both synaptic and intrinsic plasticity of layer II/III pyramidal neurons in the primary motor cortex.
Cerebral Cortex, 26: 3494-3507, 2016.

Ebrahimi M, Yamamoto Y, Sharifi K, Kida H, Kagawa Y, Yasumoto Y, Islam A, Miyazaki H, Shimamoto C, Maekawa M, Mitsushima D, Yoshikawa T, Owada Y.
Astrocyte-expressed FABP7 regulates dendritic morphology and excitatory synaptic function of cortical neurons.
Glia, 64(1):48-62, 2016.

Mitsushima D.
Contextual learning requires functional diversity at excitatory and inhibitory synapses onto CA1 pyramidal neurons.
AIMS Neuroscience, invited review 2(1):7-17, 2015.

Hosokawa T, Mitsushima D, Kaneko R, Hayashi Y.
Stoichiometry and phosphoisotypes of hippocampal AMPA-type glutamete receptor phosphorylation.
Neuron, 85(1): 60-67, 2014.

Urakawa S, Mitsushima D, Shimozuru M, Sakuma Y, Kondo Y.
An enriched rearing environment calms adult male rat sexual activity: implication for distinct serotonergic and hormonal responses to females.
PLoS ONE, 9(2):e87911, 2014.

Takase K, Sakimoto Y, Kimura F, Mitsushima D.
Developmental trajectory of contextual learning and 24-h acetylcholine release in the hippocampus.
Scientific Reports 4, 3738 doi:10.1038/ srep03738, 2014.

Mitsushima D, Sano A, Takahashi T.
A cholinergic trigger drives learning-induced plasticity at hippocampal synapses.
Nature Communications, 4:2760 doi: 10.1038/ ncomms3760, 2013.

Tada H, Kuroki Y, Funabashi T, Kamiya Y, Goto T, Suyama K, Sano A, Mitsushima D, Etgen AM, Takahashi T.
Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats.
Neuroscience, 248: 664-669, 2013.

Kikuchi T, Tan H, Mihara T, Uchimoto K, Mitsushima D, Takase K, Morita S, Goto T, Andoh T, Kamiya Y.
Effects of volatile anesthetics on the circadian rhythms of rat hippocampal acetylcholine release and locomotor activity.
Neuroscience, 237: 151-160, 2013.

Mitsushima D, Ishihara K, Sano A, Kessels HW, Takahashi T. Contaxtual learning requires synaptic AMPA receptor delivery in the hippocampus.
Proc Natl Acad Sci USA, 108,12503-12508, 2011.

Solinger JA, Paolinelli R, Kloss H, Scorza FB, Marchesi S, Sauder U, Mitsushima D, Capuani F, Sturzenbaum SR, Cassata G. The Caenorhabditis elegans Elongator complex regulates neuronal alpha-tubulin acetylation.
PLoS Genet, 6: e1000820. Jan, 2010.

Higashida T, Jitsuki S, Kubo A, Mitsushima D, Kamiya Y, Kanno H. Skin-derived precursors differentiate into dopaminergic neuronal cells in the brains of Parkinson's disease model rats.
J Neurosurg, 113:648-655, 2010.

Furuta M, Mitsushima D, Shinohara K, Kimura F, Funabashi T. Food availability affects orexin A/ hypocretin-1-induced inhibition of pulsatile luteinizing hormone secretion in female rats.
Neuroendocrinology, 91:41-47, 2010.

Hagiwara H, Kimura F, Mitsushima D, Funabashi T. Formalin-induced nociceptive behavior and c-Fos expression in middle-aged female rats.
Physiol Behav, 100:101-104, 2010.

Tin-Tin-Win-Shwe, Mitsushima D, Yamamoto S, Funabashi T, Fujimaki H. Strain differences in extracellular amino acid neurotransmitter levels in the hippocampi of MHC-congenic mice in response to toluene exposure.
Neuroimmunomodulation, 16:185-190, 2009.

Mitsushima D, Takase K, Takahashi T, Kimura F. Activational and organisational effects of gonadal steroids on sex-specific acetylcholine release in the dorsal hippocampus.
J Neuroendocrinol, invited review 21:400-405, 2009.

Tin-Tin-Win-Shwe, Hojo R, Mitsushima D, Nakajima D, Yamamoto S, Fujimaki H. Establishment of a mouse model to assess brain neurotransmitter level and learning performance simultaneously following toxic chemical exposure: using in vivo microdialysis and schedule-controlled operant behavior.
J UOEH, 31:1-11, 2009.

Takase K, Kimura F, Yagami T, Mitsushima D. Sex-specific 24-h acetylcholine release profile in the medial prefrontal cortex: simultaneous measurement of spontaneous locomotor activity in behaving rats.
Neuroscience, 159:7-15, 2009.

Jitsuki S, Kimura F, Funabashi T, Takahashi T, Mitsushima D. Sex-specific 24-h profile of extracellular serotonin levels in the medial prefrontal cortex.
Brain Res, 1260:30-37, 2009.

Mitsushima D, Takase K, Funabashi T, Kimura F. Gonadal steroids maintain 24-h acetylcholine release in the hippocampus: organizational and activational effects in behaving rats.
J Neurosci, 29:3808-3815, 2009.

Win-Shwe TT, Mitsushima D, Yamamoto S, Fujitani Y, Funabashi T, Hirano S, Fujimaki H. Extracellular glutamate level and NMDA receptor subunit expression in mouse olfactory bulb following nanoparticle-rich diesel exhaust exposure.
Inhal Toxicol, 21:828-836, 2009.

Funabashi T, Hagiwara H, Mogi K, Mitsushima D, Shinohara K, Kimura F. Sex differences in the responses of orexin neurons in the lateral hypothalamic area and feeding behavior to fasting.
Neurosci Lett, 463:31-34, 2009.

Hagiwara H, Ishida M, Arita J, Mitsushima D, Takahashi T, Kimura F, Funabashi T. The cAMP response element-binding protein in the bed nucleus of the stria terminalis modulates the formalin-induced pain behavior in the female rat.
Eur J Neurosci, 30:2379-2386, 2009.

Mitsushima D, Takase K, Funabashi T, Kimura F. Gonadal steroid hormones maintain the stress-induced acetylcholine release in the hippocampus: simultaneous measurements of the extracellular acetylcholine and serum corticosterone levels in the same subjects.
Endocrinology, 149:802-811, 2008.

Tin-Tin-Win-Shwe, Mitsushima D, Yamamoto S, Fukushima A, Funabashi T, Kobayashi T, Fujimaki H. Changes in neurotransmitter levels and proinflammatory cytokine mRNA expressions in the mice olfactory bulb following nanoparticle exposure.
Toxicol Applied Pharmacol, 226:192-198, 2008.

Takase K, Mitsushima D, Funabashi T, Kimura F. Postpubertal feeding experience affects sex-specific spatial ability in rats.
Physiol Behav, 93:553-559, 2008.

Takase K, Mitsushima D, Funabashi T, Kimura F.  Sex difference in the 24-h acetylcholine release profile in the premotor/supplementary motor area of behaving rats.
Brain Res, 1154:105-115, 2007.

Tin-Tin-Win-Shwe, Mitsushima D, Nakajima D, Ahmed S, Yamamoto S, Tsukahara S, Kakeyama M, Goto S, Fujimaki H.  Toluene induces rapid and reversible rise of hippocampal glutamate and taurine neurotransmitter levels in mice.
Toxicol Lett, 168:75-82, 2007.

Hagiwara H, Funabashi T, Mitsushima D, Kimura F.  Effects of neonatal testosterone treatment on sex differences in formalin-induced nociceptive behavior in rats.
Neurosci Lett, 412:264-267, 2007.

Fukushima A, Funabashi T, Kawaguchi M, Mitsushima D, Kimura F.  Bisphenol A induces transforming growth factor-bata3 mRNA in the preoptic area: a cDNA expression array and northern blot study.
Neurosci Lett, 411:81-85, 2007.

Mitsushima D, Yamada K, Takase K, Funabashi T, Kimura F.  Sex differences in the basolateral amygdala: the extracellular levels of serotonin and dopamine and their responses to restraint stress in rats.
Eur J Neurosci, 24:3245-3254, 2006.

Furuta M, Funabashi T, Kawaguchi M, Nakamura TJ, Mitsushima D, Kimura F.  Effects of p-nonylphenol and 4-tert-octylphenol on the anterior pituitary functions in adult ovariectomized rats.
Neuroendocrinology, 84:14-20, 2006.

Wang R, Hosaka M, Han L, Yokota-Hashimoto H, Suda M, Mitsushima D, Torii S, Takeuchi T.  Molecular probes for sensing the cholesterol composition of subcellular organelle membranes.
Biochim Biophys Acta - Mol Cell Biol Lipids, 1761:1169-1181, 2006.

Masuda J, Mitsushima D, Funabashi T, Kimura F.  Sex and housing conditions affect the 24-h acetylcholine release profile in the hippocampus in rats.
Neuroscience, 132: 537-542, 2005.

Funabashi T, Sano A, Mitsushima D, Kimura F.  Nicotine inhibits pulsatile luteinizing hormone secretion in human males but not in human females, and tolerance to this nicotine effect is lost within one week of quitting smoking.
J Clin Endocrinol Metab, 90: 3908-3913, 2005.

Mogi K, Funabashi T, Mitsushima D, Hagiwara H, Kimura F.  Sex difference in the response of melanin-concentrating hormone neurons in the lateral hypothalamic area to glucose, as revealed by the expression of phosphorylated cyclic adenosine 3',5'-monophosphate response element-binding protein.
Endocrinology, 146: 3325-3333, 2005.

Takase K, Funabashi T, Mogi K, Mitsushima D, Kimura F.  Feeding with powdered diet after weaning increases visuospatial ability in association with increases in the expression of N-methyl-d-aspartate receptors in the hippocampus of female rats.
Neurosci Res, 53:169-175, 2005.

Takase K, Mitsushima D, Masuda J, Mogi K, Funabashi T, Endo Y, Kimura F.  Feeding with powdered diet after weaning affects sex difference in acetylcholine release in the hippocampus in rats.
Neuroscience, 136: 593-599, 2005.

Kimura F, Shinohara K, Funabashi T, Daikoku S, Suyama K, Mitsushima D, Sano A.  Nicotine inhibition of pulsatile GnRH secretion is mediated by GABAA receptor system in the cultured rat embryonic olfactory placode.
Psychoneuroendocrinology, 29: 749-756, 2004.

Masuda J, Mitsushima D, Kimura F.  Female rats living in small cages respond to restraint stress both adrenocortical corticosterone release and acetylcholine release in the hippocampus.
Neurosci Lett, 358: 169-172, 2004.

Tin-Tin-Win-Shwe, Mitsushima D, Shinohara K, Kimura F.  Sexual dimorphism of GABA release in the medial preoptic area and luteinizing hormone release in gonadectomized estrogen-primed rats.
Neuroscience, 127: 243-250, 2004.

Funabashi T, Sano A, Mitsushima D, Kimura F.  Bisphenol A increases progesterone receptor immunoreactivity in the hypothalamus in a dose-dependent manner and affects sexual behaviour in adult ovariectomized rats.
J Neuroendocrinology, 15:134-140, 2003.

Mitsushima D, Tin-Tin-Win-Shwe, Kimura F.  Sexual dimorphism in the GABAergic control of gonadotropin release in intact rats.
Neurosci Res, review 46: 399-405, 2003.

Mitsushima D, Funabashi T, Shinohara K, Kimura F.  Rats living in small cages respond to restraint stress with adrenocortical corticosterone release but not with hippocampal acetylcholine release.
Psychoneuroendocrinology, 28: 574-583, 2003.

Mitsushima D, Masuda J, Kimura F.  Sex differences in the stress-induced release of acetylcholine in the hippocampus and corticosterone from the adrenal cortex in rats.
Neuroendocrinology, 78: 234-240, 2003.

Mitsushima D, Funabashi T, Kimura F.  Estrogen increases messenger RNA and immunoreactivity of aryl-hydrocarbon receptor nuclear translocator 2 in the rat mediobasal hypothalamus.
Biochem Biophys Res Commun, 307: 248-253, 2003.

Shinohara K., Funabashi T, Nakamura TJ, Mitsushima D, Kimura F.  Differential regulation of pituitary adenyl cyclase activating peptide receptor variants in the rat suprachiasmatic nucleus.
Neuroscience, 110: 301-308, 2002.

Mitsushima D. Shwe TTW, Funabashi T, Shinohara K, Kimura F.  GABA release in the medial preoptic area of cyclic female rats.
Neuroscience, 113: 109-114, 2002.

Funabashi T, Daikoku S, Suyama K, Mitsushima D, Sano A, Kimura F.  The role of gamma-aminobutyric acid (GABA) neurons in the release of gonadotropin-releasing hormone (GnRH) in cultured rat embryonic olfactory placodes.
Neuroendocrinology, 76: 193-202, 2002.

Funabashi T, Mitsushima D, Nakamura TJ, Uemura T, Hirahara F, Shinohara K, Suyama K, Kimura F.  Gonadotropin-releasing hormone (GnRH) surge generator in female rats. Elsevier Science,
Progress in Brain Res, review 141: 165-173, 2002.

Tin-Tin-Win-Shwe, Mitsushima D, Kimura F.  Profiles of in vivo GABA release in the medial preoptic area of intact and castrated male rats.
Neuroendocrinology, 76: 290-296, 2002.

Mitsushima D, Funabashi T, Shinohara K, Kimura F.  Impairment of maze learning in rats by restricting environmental space.
Neurosci Lett, 297: 73-76, 2001.

Mitsushima D, He D, Funabashi T, Shinohara K, Kimura F.  Increase in the number of detectable preoptic GAD67 immunoreactive cells in immature male rats.
Neurosci Res, 40: 141-146, 2001.

Nakamura TJ, Shinohara K, Funabashi T, Mitsushima D, Kimura F.  Circadian and photic regulation of cryptochrome mRNAs in the rat pineal gland.
Neurosci Res, 41: 25-32, 2001.

He D, Mitsushima D, Uemura T, Hirahara F, Funabashi T, Shinohara K, Kimura F.  Effects of naloxone on the serum luteinizing hormone level and the number of Fos-positive gonadotropin-releasing hormone neurons in immature female rats.
Brain Res, 858: 129-135, 2000.

Saito K, Shinohara K, Morofushi M, Funabashi T, Mitsushima D, Hata M, Matsubara S, Kimura F.  Effects of irradiation on the sensitivity of melatonin release to norepinephrine in cultured pineal bodies of rats.
Neurosci Lett, 284: 93-96, 2000.

Funabashi T, Shinohara K, Mitsushima D, Kimura F.  Estrogen increases arginine-vasopressin V1a receptor mRNA in the preoptic area of young but not of middle-aged female rats.
Neurosci Lett, 285: 205-208, 2000.

Funabashi T, Shinohara K, Mitsushima D, Kimura F.  Gonadotropin-releasing hormone exhibits circadian rhythm in phase with arginine-vasopressin in co-cultures of the female rat preoptic area and suprachiasmatic nucleus.
J Neuroendocrinol, 12: 521-528, 2000.

Shinohara K, Morofushi M, Funabashi T, Mitsushima D, Kimura F.  Effects of 5alpha-androst-16-en-3alpha-ol on the pulsatile secretion of luteinizing hormone in human females.
Chem Senses, 25: 465-467, 2000.

Shinohara K, Funabashi T, Mitushima D, Kimura F.  Effects of gap junction blocker on vasopressin and vasoactive intestinal polypeptide rhythms in the rat suprachiasmatic nucleus in vitro.
Neurosci Res, 38: 43-47, 2000.

Shinohara K, Funabashi T, Mitushima D, Kimura F.  Effects of estrogen on the expression of connexin32 and connexin43 mRNAs in the suprachiasmatic nucleus of female rats.
Neurosci Lett, 286: 107-110, 2000.

Mitsushima D, Funabashi T, Kimura F.  Fos expression in gonadotropin-releasing hormone neurons by naloxone or bicuculline in intact male rats.
Brain Res, 839: 209-212, 1999.

Keen KL, Burich AJ, Mitsushima D, Kasuya E, Terasawa E.  Effects of pulsatile infusion of the GABAA receptor blocker bicuculline on the onset of puberty in female rhesus monkeys.
Endocrinology, 140: 5257-5266, 1999.

Mitsushima D, Yamanoi C, Kimura F.  Restriction of environmental space attenuates locomotor activity and hippocampal acetylcholine release in male rats.
Brain Res, 805: 207-212, 1998.

Hla-Hla-Aye, Jinnai K, Sano A, Funabashi T, Mitsushima D, Kimura F.  Nicotine given intracerebroventricularly does not inhibit the preovulatory surge of LH and PRL secretion in female rats.
Endocine J, 45: 575-580, 1998.

Mitsushima D, Jinnai K, Kimura F.  Possible role of the gamma-aminobutyric acid-A receptor system in the timing of proestrous LH surge in rats.
Endocrinology, 138: 1944-1948, 1997.

Mitsushima D, Kimura F.  The maturation of GABAA receptor-mediated control of luteinizing hormone secretion in immature male rats.
Brain Res, 748: 258-262, 1997.

Mitsushima D, Marzban F, Luchansky LL, Burich AJ, Keen KL, Durning M, Golos TG, Terasawa E.  Role of glutamic acid decarboxylase in the prepubertal inhibition of luteinizing hormone releasing hormone release in female rhesus monkeys.
J Neurosci, 16: 2563-2573, 1996.

Mitsushima D, Mizuno T, Kimura F.  Age-related changes in diurnal acetylcholine release in the prefrontal cortex of male rats as measured by microdialysis.
Neuroscience, 72: 429-434, 1996.

Mitsushima D, Yokawa T, Nishihara M, Takahashi M.  Attenuation of the expression of circadian rhythms by chronic outputs from the VMH in rats.
Physiol Behav, 56: 891-899, 1994.

Mitsushima D, Hei DL, Terasawa E. gamma-Aminobutyric acid is an inhibitory neurotransmitter restricting the release of luteinizing hormone-releasing hormone before the onset of puberty.
Proc Natl Acad Sci USA, 91: 395-399, 1994.

Gore AC, Mitsushima D, Terasawa E.  A possible role of neuropeptide-Y in the control of the onset of puberty in female rhesus monkeys.
Neuroendocrinology, 58: 23-34, 1993.

Yokawa T, Mitsushima D, Itoh C, Konishi H, Shiota K, Takahashi M.  The ventromedial nucleus of the hypothalamus outputs long-lasting running in rats.
Physiol Behav, 46: 713-717, 1989.

Mitsushima D.  Hippocampal function and gonadal steroids.
Steroids - from physiology to clinical medicine. Edited by Prof. Sergej Ostojic, In-Tech Europe, Rijeka, Croatia. 65-82, 2012.

Mitsushima D.  Sex differences in the cholinergic system in rats: behavioural consequences.
Current Topics in Behavioral Neurosciences (CTBN) book series. Invited from Editor: Prof. Jo Neil. Springer, Heidelberg, Germany. 8:57-71, 2011.

Takase K & Mitsushima D.  Sex difference in learning and memory: effects of sex differentiation, development, and environments on the basal forebrain cholinergic system.
Horizons in Neuroscience Research, Volume 2. Invited from Editor: Dr. Andres Costa and Eugenio Villalba, Nova Science Publisher Inc, NY, USA, 47-70, 2010.

Tin-Tin-Win-Shwe & Mitsushima D.  Neural network controlling GnRH neurons in sexual dimorphism of gonadotropin release.
Current Trends in Endocrinology. Invited from Editor: Prof. Ninu Joseph. Research Trends, Kerala, India, 4, 65-75, 2010.

Mitsushima D.  Sex Steroids and Acetylcholine Release in Hippocampus.
Vitamins and Hormones - Hormones of the limbic system. Editor: Prof. Gerald Litwack. Academic Press /Elsevier, NY, USA, 82:263-277, 2010.