Jump to Research projects, Using microorganisms, Techniqus, Equipments
Microorganisms in a given environment have specific functions in response to environmental factors such as temperature and pH, as well as essential growth factors such as carbon and nitrogen sources. This function is ultimately derived from the genes encoded in its chromosome of the cells. Our goal is to discover and reveal the unique functions of various microorganisms.
Most microbial research to date has involved direct analysis or modification of specific microorganisms. Although much knowledge has been obtained by such methods, in most cases, the unique functions and mechanisms of a particular microorganism can only be utilized in its own cells. However, advances in genetic engineering have enabled large-scale genome modification of some microorganisms, making it possible to introduce and reproduce the functions (principles) of specific microorganisms. In our laboratory, we aim to understand and apply the specific function of a particular microorganism and to reproduce that function in a different microorganism. In addition, some genes are difficult to functionally express in model microorganisms. Until now, genes that do not show such functions have been left as they are, and no progress has been made in accumulating knowledge on why these genes do not function in different microorganisms. Therefore, we aim to accumulate such knowledge and technologies for improvement.
By conducting functional analysis using microorganisms, the genes are already functioning in the cells. By increasing the number of genes that function in cells and utilizing them in this way, we are working on the development of microorganisms that will make it possible to manufacture products and produce energy alone based on environmental energy using microorganisms.
Research on the unique metabolic mechanisms of microorganisms
Propionate oxidation under anaerobic conditions requires a syntrophic relationship between propionate-oxidizing bacteria and hydrogenotrophic methanogens, but the oxidation of propionate involves unique metabolic mechanisms of each microorganism. Our research focuses on the mechanism by which propionate oxidizing bacteria produce hydrogen and formic acid from propionate. On the other hand, genes of microorganisms living in extreme environments may be able to confer their unique metabolism to other microorganisms. Currently, we are attempting to express genes unique to archaea living in highly alkaline environments in Escherichia coli.
Research on metabolism and robustness of microorganisms
We are studying the robustness of microorganisms, especially their response to heat and its relationship to metabolism. We are investigating which pathways are required by E. coli for growth at high temperatures and how the intermediate metabolites synthesized by these pathways are required for growth at high temperatures. On the other hand, we are studying how to optimally construct metabolic pathways in E. coli that produce specific substances.
Research on visualization of gene function using microbial cells
Genome modification is able to be carried out process, and fluorescent proteins and tags are helpful to monitor the expression of target genes. Using these techniques, we are investigating methods to visualize internal metabolism and protein expression, especially in E. coli.