The following PhDs completed the doctor course under the suprevision of Dr. Kadokawa.
Raihana Nasrin Ferdousy
Present Position: Associate Professor of Theriogenology, Bangladesh Agriculture University
Award: Best PhD student 2022 Click link
Thesis title: Discoveries of Anti-Müllerian hormone, its receptor, and a role to stimulates
synthesis of collagen-specific chaperone HSP47 in bovine oviducts and endometria
Ferdousy RN, Suong NT, Kadokawa H. (2023) Specific locations and amounts of denatured collagen and collagen-specific chaperone HSP47 in the uterine cervices of old cows compared with those of heifers. Theriogenology 196, 10-17. Click link
Ferdousy RN, Kadokawa H. (2022) Anti-Müllerian hormone stimulates expression of the collagen-specific chaperone 47-kDa heat shock protein in bovine uterine epithelial cells. Animal Science Journal 93, e13787 Click link
Ferdousy RN, Kadokawa H. (2022) Specific locations and amounts of denatured
collagen and collagen-specific chaperone HSP47 in the oviducts and uteri
of old cows as compared with those of heifers. Reproduction, Fertility,
and Development. 34, 619-632. Click link
Abdillah DA, Kereilwe O, Ferdousy RN, Saito R, Kadokawa H. (2022) Spike
protein of SARS-CoV-2 suppresses gonadotrophin secretion from bovine anterior
pituitaries. Journal of Reproduction, and Development. 68, 152-159. Click link
Ferdousy RN, Kereilwe O, Kadokawa H. (2020) Anti-Müllerian hormone is expressed
and secreted by bovine oviductal and endometrial epithelial cells. Animal
Science Journal. 91: e13456. Click link
Ferdousy RN, Kereilwe O, Kadokawa H. (2020) Anti-Müllerian hormone receptor
type 2 (AMHR2) expression in bovine oviducts and endometria: Comparison
of AMHR2 mRNA and protein abundance between old Holstein and young and
old Wagyu females. Reproduction, Fertility, and Development 32, 738-747. Click link
Onalenna Kereilwe
Present Position: Lecturer of Animal Physiology and Embryo development, University of Botswana
Thesis title: Discoveries of anti-Müllerian hormone and anti-Müllerian hormone receptor
type 2 in bovine GnRH neurons and gonadotrophs
Message when he entered doctor course
HIs thesis has discovered that preantral and small antral follicles secret anti-Müllerian hormone (AMH) to control gonadotrophin secretion from gonadotrophs. His first part of thesis study discovered the expression of main receptor for AMH, AMH receptor type 2 (AMHR2) in both LH-gonadotrophs and FSH-gonadotrophs of postpubertal heifers to control gonadotrophin secretion. Expression of AMHR2 was detected in anterior pituitaries of postpubertal heifers using RTPCR and western blotting using our original anti-AMHR2 antibody against the extracellular region near the N-terminus of bovine AMHR2. Immunofluorescence microscopy using the same antibody visualised colocalisation of AMHR2 with gonadotrophin-releasing hormone (GnRH) receptor on the gonadotroph's special regiong of plasma membrane, lipid raft.
Furthermore, his thesis study has discovered the stimualtion effects of
AMH on LH and FSH secretion from bovine gonadotroph. Therefore, the preantral
and small antral follicles are not silent majority, and they are assiting
growth of dominant follicle! His thesis study is further advaning now!!
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Ferdousy RN, Kereilwe O, Kadokawa H. (2020) Anti-Müllerian hormone is expressed and secreted by bovine oviductal and endometrial epithelial cells. Animal Science Journal. 91: e13456. Click link
Kereilwe O, Kadokawa H. (2020) Decreased anti-Müllerian hormone and anti-Müllerian hormone receptor type 2 in hypothalami of old Japanese Black cows. Journal of Veterinary Medical Science 82, 1113-1117 Click link
Kereilwe O, Kadokawa H. (2020) Anti-Müllerian hormone and its receptor are detected in most gonadotropin-releasing-hormone cell bodies and fibers in heifer brains. Domestic Animal Endocrinology 72, 106432. Click Link
Ferdousy RN, Kereilwe O, Kadokawa H. (2020) Anti-Müllerian hormone receptor type 2 (AMHR2) expression in bovine oviducts and endometria: Comparison of AMHR2 mRNA and protein abundance between old Holstein and young and old Wagyu females. Reproduction, Fertility, and Development 32, 738-747. Click link
Kereilwe O, Kadokawa H. (2019) Bovine gonadotrophs express anti-Müllerian hormone (AMH): Comparison of AMH mRNA and protein expression levels among old Holsteins and young and old Japanese black females. Reproduction, Fertility, and Development 31, 810-819. Click Link
Kereilwe O, Pandey K, Kadokawa H.(2018) Influence of brain plasmalogen on gonadotropin secretion from the cultured bovine anterior pituitary cells. Domestic Animal Endocrinilogy 64, 77-83. Click Link
Kadokawa H. Pandey K, Kereilwe O, Nahar A. (2018) Reconsidering the roles of endogenous estrogens and xenoestrogens: the membrane estradiol receptor G protein-coupled receptor 30 (GPR30) mediates the effects of various estrogens. Journal of Reproduction, and Development 64, 203-208. Click Link
Kereilwe O, Pandey K, Borromeo V, Kadokawa H. (2018) Anti-Müllerian hormone receptor type 2 is expressed in gonadotrophs of post-pubertal heifers to control gonadotropin secretion. Reproduction, Fertility, and Development 30, 1192-1203. Click Link
Pandey K, Kereilwe O, Kadokawa H. (2018) Heifers express G-protein coupled receptor 153 in anterior pituitary gonadotrophs in stage-dependent manner. Animal Science Journal 89, 60-71. Click Link
Kiran Pandey
THESIS TITLE: Global gene expression analyses and discovery of new receptors in bovine anterior pituitary.
Present Position: Researcher, Department of Livestock Services, Nepal government
Message when he graduated: "Beautiful memories in Yamaguchi "
We have found several new important receptors expressed in anterior pituitary
utilzing next generation sequence etc.
The rexpression levels of some receptors (e.g., GPR61) are increased in estrus!!
GPR61 colocalizes with GnRH receptor on the surface of gonadotorph as shown
in the immunofluoresence photo.
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Pandey K, Kereilwe O, Kadokawa H. (2018) Heifers express G-protein coupled receptor 153 in anterior pituitary gonadotrophs in stage-dependent manner. Animal Science Journal (in press)
Pandey K, Mizukami Y, Watanabe K, Sakaguti S, Kadokawa H. (2017) Deep sequencing
of the transcriptome in the anterior pituitary of heifers before and after
ovulation. Journal of Veterinary Medical Science, 79(6):1003-1012
Pandey K, Kereilwea O, Borromeo V, Kadokawa H. (2017) Heifers express G-protein
coupled receptor 61 in anterior pituitary gonadotrophs in stage-dependent
manner. Animal Reproduction Science , 181: 93-102.
Pandey K, Ashrafun Nahar A, Kadokawa H. (2016) Method for isolating pure bovine gonadotrophs from anterior pituitary using magnetic nanoparticles and anti-gonadotropin-releasing hormone receptor antibody. Journal of Veterinary Medical Science 78, 1699-1702.
Nakamura U, Rudolf FO , Pandey K, Kadokawa H. (2015) The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner. Animal Reproduction Science 156, 118-127.
Kadokawa H, Pandey K, Nahar A, Nakamura U, Rudolf FO. (2014) Gonadotropin-releasing hormone (GnRH) receptors of cattle aggregate on the surface of gonadotrophs and are increased by elevated GnRH concentrations. Animal Reproduction Science 150, 84-95.
Asrafun Nahar
Thesis Title: Effects of nutritional condition or estrus cycle on GMCSF
and MIF expression in bovine oviducts.
Present Position: Researcher in Department of Molecular, Cellular and Developmental Biology, Colorado University,
USA.
Message when she graduated: click
Nahar A, Maki S, Kadokawa H. (2013). Suppressed expression of granulocyte
macrophage colony-stimulating factor in oviduct ampullae of obese cows.
Animal Reproduction Science 139, 1-8. LINK
| POINT |
| The expression of GMCSF, the important protein controlling embryogenesis is suppressed in ampulla part of oviduct of obese cow. |
Nahar A, Kadokawa H. (2016) Suppressed expression of macrophage migration
inhibitory factor in the oviducts of lean and obese cows. Reproduction,
Fertility, and Development (in press) doi: 10.1071/RD14164 LINK
Photo is used in the cover of Issue 6 of Reproduction, Fertility and Development
as above photo!!
Nahar A, Kadokawa H. (2017) Expression of macrophage migration inhibitory
factor (MIF) in bovine oviducts is higher in the postovulatory phase than
during oestrus and the luteal phase. Reproduction, Fertility, and Development.
(in press)
Kadokawa H, Pandey K, Nahar A, Nakamura U, Rudolf FO. (2014) Gonadotropin-releasing hormone (GnRH) receptors of cattle aggregate on the surface of gonadotrophs and are increased by elevated GnRH concentrations. Animal Reproduction Science 150, 84-95.
Koizumi M, Nahar A, Yamabe R, Kadokawa H. (2016) Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. Journal of Reproduction and Development. 62, 257-263.
Pandey K, Ashrafun Nahar A, Kadokawa H. (2016) Method for isolating pure bovine gonadotrophs from anterior pituitary using magnetic nanoparticles and anti-gonadotropin-releasing hormone receptor antibody. Journal of Veterinary Medical Science 78, 1699-1702.
Faidiban Oktofianus Rudolf
Thesis Title:Rapid non-genomic control by membrane estradiol receptor,
GPR30, for luteinizing hormone secretion from bovine anterior pituitary.
Present Position: Professor of West Papua State University in Indonesia
Publication List:
Rudolf FO, Kadokawa H (2013). Expression of estradiol receptor, GPR30,
in bovine anterior pituitary and effects of GPR30 agonist on GnRH-induced
LH secretion. Animal Reproduction Science 139, 9-17. LINK
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| We discovered a new estradiol receptor, GPR30, controllig luteinizing hormone secretion in bovine pituitary. Also various important agonists have been discovered by our group. |
Rudolf FO, Kadokawa H (2014) Effects of STX, a Novel Estrogen Membrane Receptor Agonist, on GnRH-Induced Luteinizing Hormone Secretion from Cultured Bovine Anterior Pituitary Cells. Journal of Veterinary Medical Science 76, 1623-1625. LINK
Kadokawa H, Pandey K, Nahar A, Nakamura U, Rudolf FO (2014) Gonadotropin-releasing
hormone (GnRH) receptors of cattle aggregate on the surface of gonadotrophs
and are increased by elevated GnRH concentrations. Animal Reproduction
Science 150, 84-95. LINK
Nakamura U, Rudolf FO , Pandey K, Kadokawa H (2015) The non-steroidal mycoestrogen
zeranol suppresses luteinizing hormone secretion from the anterior pituitary
of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner.
Animal Reproduction Science 156, 118-127. LINK
Rudolf FO, Kadokawa H (2016). Cytoplasmic kinases downstream of GPR30 suppress
GnRH-induced LH secretion from bovine anterior pituitary cells. Journal
of Reproduction and Development (in press) LINK