腎班研究
腎移植に関する研究
これまでに、tacrolimusやsteroid、FTY720などの免疫抑制剤のＴリンパ球（胸腺）に及ぼす影響や[1-6]、Ｔリンパ球の細胞内情報伝達、特にT cell receptorを介するco-stimulatory signalの情報伝達機構に関する研究を行ってきた[7-14]。
最近ではGGAがheat shock proteinを誘導し、腎虚血再灌流障害を軽減する可能性があることを確認している。現在、脾臓摘出の腎虚血再灌流障害抑制効果に関する研究が進行中である。
腹膜透析に関する研究
これまでに培養腹膜中皮細胞を用いた腹膜障害機序に関する研究[15]、Interleukin-1 receptor antagonistの遺伝子多型が腹膜透析時の腹膜炎発症に及ぼす影響[16]などを研究してきた。最近、ラット腹膜硬化症モデルの作成[17]を行い、現在このモデルを用いた研究が進行中である。
1. Takai K., Tsuchida M., Konishi M., Tokuda N., Fujikura Y., jojima K., and Fukumoto T. Immunosuppressive effects of FK506 on rat lymphoid organs. Transplant. Proc., 23: 2964-2966, 1991.
２. Tsuchida M., Konishi M., Jojima K., Naito K., Fujikura Y., and Fukumoto T. Analysis of cell surface antigens on glucocorticoid-treated rat thymocytes with monoclonal antibodies. Immunol. Lett., 39: 209-217, 1994.
3. Tsuchida M., Takai K., Jojima K., Naito K., Fujikura Y., and Fukumoto T. The effects of FK506 and dexamethasone on rat thymocyte differentiation. Ther. Immunol., 1: 135-141, 1994.
4. Tsuchida M., Konishi M., Takai K., Naito K., Fujikura Y., and Fukumoto T. Effects of irradiation, glucocorticoid and FK506 on cell-surface antigen expression by rat thymocytes: a three-color flow cytofluorometric analysis. Immunol., 83: 469-476, 1994.
5. Tsuchida M. Rat CD4- CD8- TCRhigh thymocytes. Bull. Yamaguchi Med. Sch., 43:3-4, 1996.
6. Isoyama N., Takai K., Tsuchida M., Matsumura M., andNaito K. Evidence that FTY720 induces rat thymocyte apoptosis. [Journal Article] Transplant Immunol. 15(4):265-71, 2006 .
7. Tsuchida M., Knechtle SJ., and Hamawy MM. CD28 ligation induces tyrosine phosphorylation of Pyk2 but not Fak in Jurkat T cells. J. Biol. Chem., 274: 6735-6740, 1999.
8. Hamawy MM., Tsuchida M., Fechner JH., Manthei ER., and Knechtle SJ. Involvement of protein tyrosine phosphorylation in immunotoxin effects in T lymphocytes. Transplant. proc., 31: 785, 1999.
9. Tsuchida M., Manthei ER., Knechtle SJ., and Hamawy MM. LAT is tyrosine phosphorylated upon CD28 ligation. Eur. J. Immunol., 29: 2354-2359, 1999.
10. Hamawy MM., Tsuchida M., Manthei E., Dong Y., Fechner JH., and Knechtle SJ. 1999. Activation of T lymphocytes for adhesion and cytokine expression by toxin-conjugated anti-CD3 monoclonal antibodies. Transplantation, 68: 693-698, 1999.
11. Tsuchida M., Manthei ER., Knechtle SJ., and Hamawy MM. T cell activation upregulates the expression on the focal adhesion kinase Pyk2: Opposing roles for the activation of protein kinase C and the increase in intracellular Ca2+. J. Immunol., 163: 6640-6650, 1999.
12. Tsuchida M., Manthei ER., Tausif A., Knechtle SJ., and Hamawy MM. Regulation of T cell receptor- and CD28-induced tyrosine phosphorylation of the focal adhesion tyrosine kinases Pyk2 and Fak by protein kinase C. A role for protein tyrosine phosphatases. J. Biol. Chem., 275:1344-1350, 2000.
13. Peters D., Tsuchida M., Manthei ER., Tausif A., Cho CS., Knechtle SJ., and Hamawy MM. Potentiation of CD3-induced expression of the linker for activation of T cells (LAT) by the calcineurin inhibitors cyclosporin A and FK506. Blood. 95: 2733-2741, 2000.
14. Tsuchida M., Takai K., Hamawy MM., Knechtle SJ., Naito K. Involvement of linker for activation of T cells in the costimulatory signaling pathways. Transplant. Proc., 35(1):553-4, 2003.
15. Fujikawa, K., Takai, K., Suga, A., Naito, K., Ohata, A., Nakasone, S., Horiuchi, T.
Expression of mRNA for growth factors and extracellular matrix proteins after injury to cultured peritoneal cells: does the healing process contribute to peritoneal ultrastructural alteration? The International Journal of Artificial Organs, 6, 253-259, 2003.
16. Uchiyama K., Naito K., Tsuchida M., Takai K., Okayama N., Fujimura K. Hinoda Y. Impact of a genetic polymorphism of the interleukin-1 receptor antagonist on technique survival in peritoneal dialysis patients. Blood Purification. 23(6):450-8, 2005.
17. Komatsu H., Uchiyama K., Tsuchida M., Isoyama N., Matsumura M., Hara T., Fukuda M., Kanaoka Y., and Naito K. Development of a peritoneal sclerosis rat model using a continuous-infusion pump. Perit. Dial. Int. 28: 641-647, 2008.
